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Antonie van Leeuwenhoek (2014) 106:489495 DOI 10.1007/s10482-014-0217-1
ORIGINAL PAPER
Sporosarcina siberiensis sp. nov., isolated from the East Siberian Sea
Gaiyun Zhang Huihui Ren Xiu Chen
Yubian Zhang Yanliu Yang Shuang Wang
Yi Jiang
Received: 11 April 2014 / Accepted: 12 June 2014 / Published online: 3 July 2014 Springer International Publishing Switzerland 2014
Abstract A Gram-stain positive, non-motile, rod-shaped bacterium, designated strain 1111S-42T, was isolated from the East Siberian Sea. The organism was found to grow at 430 C, pH 7.08.5 and in 08 % (w/v) NaCl, with optimal growth occurring at 28 C, pH 7.5 and in 1 % NaCl. Based on 16S rRNA gene sequence similarity studies, strain 1111S-42T was found to belong to the genus Sporosarcina and to be most closely related to Sporosarcina contaminans CCUG53915T (97.3 %) and Sporosarcina soli I80T(97.2 %). The main polar lipids were found to include diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant menaquinone was identied as MK-7. The major cellular fatty acids were identied as anteiso-C15:0 (34.4 %), iso-C15:0
(29.8 %) and anteiso-C17:0 (22.4 %). The DNA G?C content of strain 1111S-42T was determined to be 39 mol %. The values of DNADNA relatedness
between the strain 1111S-42T and related type strains of the genus Sporosarcina were less than 30 %. Based on the phylogenetic analysis, along with extensive physiological and chemotaxonomic testing, we conclude that the bacterium represents a novel species of the genus Sporosarcina, for which the name Sporosarcina siberiensis sp. nov. is proposed. The type strain is strain 1111S-42T (=CGMCC 1.12516T = LMG 27494T).
Keywords Sporosarcina siberiensis sp. nov.
Novel species Polyphasic taxonomy Sediment
Introduction
The genus Sporosarcina, belonging to the family Bacillaceae, was rst described by Kluyver and van Niel (1936). Sporosarcina species have been isolated from diverse sources including sea water, sediment, soil, and human blood, along with built environments such as industrial oors and soy sauce production equipment. At the time of writing, the genus contains 12 validly named species: Sporosarcina ureae (Kluyver and van Niel 1936), Sporosarcina globispora (Larkin and Stokes 1967; Yoon et al. 2001), Sporosarcina pasteurii (Chester 1898; Yoon et al. 2001), Sporosarcina psychrophila (Nakamura 1984; Yoon et al. 2001), Sporosarcina aquimarina (Yoon et al. 2001), Sporosarcina koreensis (Kwon et al. 2007), Sporosarcina soli (Kwon et al. 2007),...