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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Phosphate is a major plant macronutrient and low phosphate availability severely limits global crop productivity. In Arabidopsis, a key regulator of the transcriptional response to low phosphate, phosphate starvation response 1 (PHR1), is modulated by a class of signaling molecules called inositol pyrophosphates (PP-InsPs). Two closely related diphosphoinositol pentakisphosphate enzymes (AtVIP1 and AtVIP2) are responsible for the synthesis and turnover of InsP8, the most implicated molecule. This study is focused on characterizing Arabidopsis vip1/vip2 double mutants and their response to low phosphate. We present evidence that both local and systemic responses to phosphate limitation are dampened in the vip1/vip2 mutants as compared to wild-type plants. Specifically, we demonstrate that under Pi-limiting conditions, the vip1/vip2 mutants have shorter root hairs and lateral roots, less accumulation of anthocyanin and less accumulation of sulfolipids and galactolipids. However, phosphate starvation response (PSR) gene expression is unaffected. Interestingly, many of these phenotypes are opposite to those exhibited by other mutants with defects in the PP-InsP synthesis pathway. Our results provide insight on the nexus between inositol phosphates and pyrophosphates involved in complex regulatory mechanisms underpinning phosphate homeostasis in plants.

Details

Title
A Role for Inositol Pyrophosphates in the Metabolic Adaptations to Low Phosphate in Arabidopsis
Author
Land, Eric S 1 ; Cridland, Caitlin A 2 ; Branch Craige 2 ; Dye, Anna 1 ; Hildreth, Sherry B 2 ; Helm, Rich F 2   VIAFID ORCID Logo  ; Gillaspy, Glenda E 2 ; Perera, Imara Y 1   VIAFID ORCID Logo 

 Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA; esland@ncsu.edu (E.S.L.); aedye@ncsu.edu (A.D.) 
 Department of Biochemistry, Virginia Polytechnic and State University, Blacksburg, VA 24061, USA; cridc2@vt.edu (C.A.C.); craigeb@vt.edu (B.C.); shildret@vt.edu (S.B.H.); helmrf@vt.edu (R.F.H.); gillaspy@vt.edu (G.E.G.) 
First page
601
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
22181989
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2576451356
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.