Abstract

Auxin critically regulates plant growth and development. Auxin-driven transcriptional responses are mediated through the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. ARF protein condensation attenuates ARF activity, resulting in dramatic shifts in the auxin transcriptional landscape. Here, we perform a forward genetics screen for ARF hypercondensation, identifying an F-box protein, which we named AUXIN RESPONSE FACTOR F-BOX1 (AFF1). Functional characterization of SCFAFF1 revealed that this E3 ubiquitin ligase directly interacts with ARF19 and ARF7 to regulate their accumulation, condensation, and nucleo-cytoplasmic partitioning. Mutants defective in AFF1 display attenuated auxin responsiveness, and developmental defects, suggesting that SCFAFF1 -mediated regulation of ARF protein drives aspects of auxin response and plant development.

Auxin-driven transcriptional responses are mediated by ARF transcription factors. Here the authors characterize an F-box protein, AFF1, that regulates the accumulation, condensation, and nucleo-cytoplasmic partitioning of ARF19 and ARF7.

Details

Title
Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning
Author
Jing, Hongwei 1   VIAFID ORCID Logo  ; Korasick, David A. 2   VIAFID ORCID Logo  ; Emenecker, Ryan J. 3 ; Morffy, Nicholas 1 ; Wilkinson, Edward G. 1 ; Powers, Samantha K. 2 ; Strader, Lucia C. 1   VIAFID ORCID Logo 

1  Duke University, Department of Biology, Durham, USA (GRID:grid.26009.3d) (ISNI:0000 0004 1936 7961); Washington University, Center for Engineering MechanoBiology, St. Louis, USA (GRID:grid.4367.6) (ISNI:0000 0001 2355 7002); Washington University, Center for Science and Engineering Living Systems (CSELS), St. Louis, USA (GRID:grid.4367.6) (ISNI:0000 0001 2355 7002) 
2  Washington University, Department of Biology, St. Louis, USA (GRID:grid.4367.6) (ISNI:0000 0001 2355 7002) 
3  Washington University, Center for Engineering MechanoBiology, St. Louis, USA (GRID:grid.4367.6) (ISNI:0000 0001 2355 7002); Washington University, Center for Science and Engineering Living Systems (CSELS), St. Louis, USA (GRID:grid.4367.6) (ISNI:0000 0001 2355 7002); Washington University, Department of Biology, St. Louis, USA (GRID:grid.4367.6) (ISNI:0000 0001 2355 7002) 
Publication year
2022
Publication date
2022
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-022-31628-2
ProQuest document ID
2687919057
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.