Regeneration of Blackgram (Vigna mungo L.) on Changes of Hormonal Condition

Abstract

The study comprised of experiments for shoot regeneration and plantlet formation from cotyledonary node of Vigna mungo by culturing them on low concentration of BAP followed by transfer to hormone free MS medium. Cotyledonary node explants were cultured on different concentrations of BAP (0, 1, 2.5 and 50 mg l-1). Shoot regeneration occurred from cotyledonary nodes irrespective of the presence or absence of BAP in the medium. However, culture of cotyledonary node explants for 10 days on medium containing 1.0 mg l-1 BAP followed by transfer to hormone free medium gave higher number of shoots (9.33/explant) compared to culture of the explants on hormone free medium for 15 days followed by transfer to medium containing 1.0 mg l-1 BAP (8.33/explants). The regenerated shoots were transferred to rooting medium supplemented with different concentrations of IBA and NAA. The high frequency (100.0%) of rooting was observed with MS medium supplemented with 0.5 mg l-1 IBA. The rooted plants were transferred to pots for hardening.