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Immunol Res (2014) 58:5160 DOI 10.1007/s12026-013-8479-7
Induction of bone loss in DBA/1J mice immunizedwith citrullinated autologous mouse type II collagen in the absence of adjuvant
Anand Dusad Michael J. Duryee Anita T. Shaw Lynell W. Klassen
Daniel R. Anderson Dong Wang Ke Ren Ellen M. Gravallese
James R. ODell Ted R. Mikuls Geoffrey M. Thiele
Published online: 27 December 2013 Springer Science+Business Media New York 2013
Abstract Joint damage in rheumatoid arthritis (RA) is characterized by cartilage and bone loss resulting in pain, deformity, and loss of joint function. Anti-citrullinated protein antibody (ACPA) has been implicated in RA pathogenesis and predicts radiographical joint damage and clinical severity. Therefore, the purpose of this study was to assess bone loss by micro-CT, histological joint damage, and ACPA levels using a mouse model of RA. Arthritis was induced by immunizing DBA/1 mice with autologous citrullinated type II mouse collagen (CIT-CII) weekly for 4 weeks. Mice immunized with autologous CII served as
controls. At week 5, mice were killed, ACPA levels determined, and micro-CT performed to quantitatively analyze bone damage. Micro-CT analysis revealed significant loss of bone density, volume, and surface (p \ 0.05)
in bone peripheral to the inamed joints of CIT-CII animals compared to CII controls. Histological staining demonstrated cartilage, proteoglycan, joint collagen, and bone collagen loss in the CIT-CII group compared to CII. Serum ACPA levels were increased (p = 0.03) in the CITCII group compared to CII, and these levels were inversely correlated with bone quantity and quality. In this study, we
A. Dusad M. J. Duryee (&) L. W. Klassen
J. R. ODell T. R. Mikuls G. M. Thiele
Experimental Immunology Laboratory, Omaha Veterans Administration Medical Center, Omaha, NE 68105, USA e-mail: [email protected]
A. Dusade-mail: [email protected]
L. W. Klassene-mail: [email protected]
J. R. ODelle-mail: [email protected]
T. R. Mikulse-mail: [email protected]
G. M. Thielee-mail: [email protected]
A. Dusad M. J. Duryee L. W. Klassen
J. R. ODell T. R. Mikuls G. M. Thiele
Experimental Immunology Laboratory, Division of Rheumatology, Department of Internal Medicine, University of Nebraska Medical Center, Wittson Hall Room 3005, Omaha, NE 68198-6350, USA
A. T. Shaw E. M. Gravallese
Department of Medicine, Division of Rheumatology, University of Massachusetts Medical School, Worcester, MA 01605, USA e-mail: [email protected]
E. M. Gravallesee-mail: [email protected]
D. R. AndersonExperimental Immunology Laboratory,...