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Rheumatol Int (2014) 34:12631265 DOI 10.1007/s00296-013-2936-4
LETTER TO THE EDITOR
Idiopathic retroperitoneal brosis: IgG4 inltration in a cohort of Spanish patients
Andreu FernndezCodina Fernando MartnezValle Jess CastroMarrero Ins De Torres
Miquel VilardellTarrs Jos OrdiRos
A. Fernndez-Codina F. Martnez-Valle M. Vilardell-Tarrs J. Ordi-Ros
Systemic Autoimmune Disease Unit, Department of Internal Medicine, Hospital Universitari Vall dHebron, Universitat Autnoma de Barcelona, 3 Planta Edicio Hospital General, Passeig de la Vall dHebron 119-129, 08035 Barcelona, Spain e-mail: [email protected]
A. Fernndez-Codina F. Martnez-Valle J. Castro-Marrero M. Vilardell-Tarrs J. Ordi-Ros
Systemic Autoimmune Disease Research Unit, Vall dHebron Institute of Research, Hospital Universitari Vall dHebron, Universitat Autnoma de Barcelona, Barcelona, Spain
F. Martnez-Valle (*)
Hospital Universitari Vall dHebron, Servicio de Medicina Interna, Unidad de Enfermedades Autoinmunes Sistmicas, 3 Planta Edicio Hospital General, Passeig de la Vall dHebron 119-129, 08029 Barcelona, Spaine-mail: [email protected]
I. De Torres
Pathology Department, Hospital Universitari Vall dHebron, Universitat Autnoma de Barcelona, Barcelona, Spain
Received: 18 September 2013 / Accepted: 25 December 2013 / Published online: 10 January 2014 Springer-Verlag Berlin Heidelberg 2014
Sections were stained with haematoxylin and eosin as well as for Massons trichrome. Immunostaining for IgG4 was performed on 4-m-thick parafn-embedded human surgical biopsy sections using as the primary antibody a mouse anti-human IgG4 monoclonal antibody (clone MC011, The Binding Site, Birmingham, UK). Sections were also immunostained for anti-CD4 (clone 4B12, Master Diagnostica, Granada, Spain; commercial pre-concentrated dilution 1:2) and anti-CD8 (clone C8/144B, Dako Cytomation, Glostrup, Denmark; dilution 1:50) antibodies using an autostainer (Bond-Max Leica, Wetzlar, Germany). Immunohistochemistry slides were evaluated by two independent pathologists; discordant cases were re-evaluated. IgG4-positive plasmacytes were counted in 3 different high-power elds (HPF) at 400 for each
specimen in the most prominently inamed areas, and the results were compared. The stained cell recount was performed using Soft Imagin System Cell-B (Olympus, Spain).
Nineteen patients were male. All patients were of Caucasian race/ethnicity except a North African subject. Mean standard deviation age at diagnosis was