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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

The GLK gene family is of great significance in regulating chloroplast development and participating in chlorophyll synthesis. However, the mechanism of GLK involvement in Citrus chlorophyll synthesis remains unclear. In this study, bioinformatics methods were used to analyze the gene structure, protein evolution, chromosome distribution, promoter elements and expression profile of GLK gene family in Citrus. Overall, 27 CsGLK TFs were identified from Citrus genome and divided into three subgroups according to the conserved domains. All members were distributed on nine chromosomes. The tandem replication events (ka/ks < 1) indicated that CsGLK TFs underwent a purification selection evolutionary process. The intron variation might be a vital configuration for the evolution of CsGLK genes. The expression pattern of CsGLKs showed that family members had higher expression levels in different tissues and at different growth stages and could actively respond to dark stress. CsGLK TFs of the same group had similar structures, but their expression patterns were quite different, indicating that they may have different functions and not be redundant. Correlation analysis showed that CsGLK2, CsGLK9, CsGLK10, CsGLK11, CsGLK20 and CsGLK24 were significantly positive correlations with Chl a and Chl b contents. In addition, CsGLK2, CsGLK5, CsGLK10, CsGLK11, CsGLK12, CsGLK15, CsGLK20 and CsGLK24 were significantly positive related to Mg-Proto IX, Proto IX and Pchl.

Details

Title
Genome-Wide Analysis of the GLK Gene Family and the Expression under Different Growth Stages and Dark Stress in Sweet Orange (Citrus sinensis)
Author
Xiong, Bo 1   VIAFID ORCID Logo  ; Gong, Yan 1 ; Li, Qin 1 ; Li, Ling 1 ; Mao, Huiqiong 1 ; Liao, Ling 1   VIAFID ORCID Logo  ; Wang, Xun 2 ; Deng, Honghong 2 ; Zhang, Mingfei 1 ; Wang, Zhihui 2 

 College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China 
 Institute of Pomology and Olericulture, College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China 
First page
1076
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
23117524
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2748281494
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.