The GAPDH partial gene of durik-durik (Syzygium sp.) from Riau Province of Indonesia

Abstract

GAPDH gene encoding glyceraldehyde-3-phosphate dehydrogenase enzyme is one of housekeeping genes which is often used as an internal conntrol in gene expression analysis. This study is aimed to isolate the GAPDH gene from durik-durik (Syzygium sp.). Fresh leaves of Syzygium sp. were collected from Kajuik Lake which is located in Langgam District, Pelalawan Regency, Riau Province, Indonesia. The methods included total DNA isolation using plant DNA isolation kit (Qiagen), polymerase chain reaction, electrophoresis, and sequencing. Data was analyzed using BioEdit7 and MEGA6 softwares, and BLAST program. The DNA partial sequence of GAPDH gene was obtained from Syzygium sp. with the length of 623 bp. The DNA sequence was predicted consisting of 2 exons and 3 introns and encoding 68 amino acids. The DNA sequence of Syzygium sp. GAPDH gene had 74%-89% similartiy to one of other plant species, but none was member of Syzygium genus. The deduced amino acid sequence had 100% similartiy to one of Tropaeolum minus dan Mentha spicata plants. The GAPDH partial gene obtained in this study is the first one reported from Syzygium genus and this sequence can be a basis for isolating of GAPDH gene from other species in Syzygium genus.