Continuity of Procambium and Anomalous Cambium During Formation of Successive Cambia in Celosia argentea

Abstract

The development of woody plants is related to the continuity of the procambium and cambium. Whether such a continuity is present in plants with successive cambia, especially in those, where the first cambium is formed outside the primary vascular bundles, has not been analyzed so far. Therefore, we studied the development of vascular meristem in Celosia argentea, in which the first and successive cambial cylinders arise outside the primary bundles and, intriguingly, in the literature are interpreted as developmentally independent structures. Our results showed that in C. argentea, the outermost procambial cells maintain their meristematic characteristics during differentiation of vascular bundles and divide periclinally, forming the zone of procambium-derived cells outside the primary bundles. This zone comprises parenchyma cells bordering the bundles, and a continuous ring of the incipient cambial cells neighboring the primary cortex. Later in the development, the ability to preserve the outermost cells in the cambium undifferentiated is repeated during the formation of successive cylinders of cambia. Together, our results clearly point to the developmental continuity of the procambium and successive cambia in C. argentea, despite their seemingly spatial distinctiveness. We postulate that the mechanism demonstrated in C. argentea is universal and orchestrates the development of successive cambia in other plant species.