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Mol Biol Rep (2010) 37:825832 DOI 10.1007/s11033-009-9628-x
Composition and phylogenetic analysis of wheat cryptochrome gene family
Pei Xu Hui Lan Zhu Hai Bin Xu Zheng Zhi Zhang Cai Qin Zhang Li Xia Zhang Zheng Qiang Ma
Received: 1 February 2009 / Accepted: 9 July 2009 / Published online: 22 July 2009 Springer Science+Business Media B.V. 2009
Abstract Cryptochrome (CRY) gene family encodes photoreceptors mediating developmental responses to blue light throughout the life of plants. We report here the characterization of CRY gene family in hexaploid wheat. Degenerate PCR amplication of the regions encoding the conserved avin-binding domain of CRY proteins yielded seven bands, resulting from amplication of CRY1a, CRY1b and CRY2 homologous genes. Assignment of individual amplicons to subgenomes was accomplished by comparing their sequence compositions with those from the ancestor species of wheat. ESTs coding for CRY-DASH like proteins were identied in wheat EST database
in GenBank. Southern blot showed that TaCRY1a, TaCRY1b and TaCRY2 are single copy genes. We mapped TaCRY1a and TaCRY2 to chromosomes of homoeologous group 6, TaCRY1b to group 2, and TaCRY-DASH to group7. Phylogenetic analysis showed that CRY subfamily diversication occurred before the divergence of monocots and dicots. The regulatory and functional changes of CRY members within subfamily are discussed.
Keywords Cryptochrome Gene family Phylogeny
Wheat
AbbreviationsCRY CryptochromeEST Expressed sequence tag PCR Polymerase chain reaction PHR Photolyase related
Electronic supplementary material The online version of this article (doi:http://dx.doi.org/10.1007/s11033-009-9628-x
Web End =10.1007/s11033-009-9628-x ) contains supplementary material, which is available to authorized users.
P. Xu H. L. Zhu H. B. Xu Z. Z. Zhang
C. Q. Zhang L. X. Zhang Z. Q. Ma
The Applied Plant Genomics Lab, Crop Genomicsand Bioinformatics Center, Nanjing Agricultural University, 210095 Jiangsu, Peoples Republic of China
P. Xu H. L. Zhu H. B. Xu Z. Z. Zhang
C. Q. Zhang L. X. Zhang Z. Q. Ma
National Key Lab of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095 Jiangsu, Peoples Republic of China
Z. Q. Ma (&)
College of Agricultural Sciences, Nanjing Agricultural University, 210095 Nanjing, Jiangsu, Peoples Republic of Chinae-mail: [email protected]
Present Address:P. XuInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences, 310021 Hangzhou, Peoples Republic of China
Introduction
Cryptochromes (CRYs) belong to the blue light-absorbing CRYs/photolyase superfamily that also includes DNA repairing enzyme photolyases...