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Antonie van Leeuwenhoek (2014) 105:623639 DOI 10.1007/s10482-013-0099-7
REVIEW PAPER
Brevibacillus as a biological tool: a short review
Amrita Kumari Panda Satpal Singh Bisht
Surajit DeMondal N. Senthil Kumar
G. Gurusubramanian Ashok Kumar Panigrahi
Received: 19 September 2013 / Accepted: 11 December 2013 / Published online: 22 February 2014 Springer Science+Business Media Dordrecht 2014
Abstract The signicance of Brevibacillus has been documented scientically in the published literature and commercially in heterologous recombinant protein catalogs. Brevibacillus is one of the most widespread genera of Gram-positive bacteria, recorded from the diverse environmental habitats. The high growth rate, better transformation efciency by electroporation, availability of shuttle vectors, production of negligible amount of extracellular protease, and the constitutive expression of heterologous proteins make some strains of this genus excellent laboratory models. Regarding biotechnological applications, this genus continues to be a source of various enzymes of great biotechnological interest due to their ability to bio-degrade low density polyethylene, ability to act as a candidate bio-control agent, and more recently acknowledged as a tool for the overexpression. This article reviews the properties of Brevibacillus spp. as better biological tools with varied applications.
Keywords Brevibacillus Biotechnology
Laboratory tool Heterologous expression
Introduction
The genus Brevibacillus was established in the year 1996, derived by a genetic reclassication of strains previously allotted to the Bacillus brevis group.B. brevis was rst described in (Migula 1900) and reclassied as a species belonging to the novel genus Brevibacillus, along with nine other species (Shida et al. 1996). Results of a gene sequence study by Shida et al. (1996) demonstrated that the B. brevis cluster includes ten species, i.e., B. brevis, B. agri, B. centrosporus,B.choshinensis, B. parabrevis, B. reuszeri, B. formosus,B. borstelensis, B. laterosporus, and B. thermoruber. Currently, the genus Brevibacillus includes 20 species (Fig. 1; Table 1) with published names (http://www.bacterio.cict.fr/b/Brevibacillus.html
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The genus Brevibacillus
The genus Bacillus is a phenotypically large, diverse collection of Gram-positive or Gram-variable, endo-spore-forming, aerobic, facultative anaerobic, and rod-shaped bacteria that have undergone considerable reclassication along with the advances in molecular biology with a high phylogenetic heterogeneity (Claus
A. K. Panda (&) S. S. Bisht S. DeMondal
N. Senthil KumarDepartment of Biotechnology, Mizoram University, Aizwal 796004, Mizoram, Indiae-mail: [email protected]
G. GurusubramanianDepartment of Zoology, Mizoram University, Aizwal 796004,...