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ABSTRACT
A total of 24 ejaculates comprising 6 ejaculates from each of four trained healthy crossbred Hampshire boars of one to two years of age were selected for the present study. Semen was collected by Simple fist method to study the effect of Modena extender on quality of semen during preservation at 15 °C up to 120 hours. The results revealed that the sperm motility, live intact acrosome, HOST-reacted spermatozoa, sperm membrane protein, sperm cholesterol level decreased significantly (P<0.01) with increase in hour of preservation. The highest conception rate (93.33%) was observed at immediately after extension and lowest conception (40%) was observed at 120 hours of preservation. In conclusion, the boar spermatozoa undergo several changes including diminished motility, viability and alterations in membrane permeability during long term storage at 15°C with Modena extender.
Keywords: Boar, semen, liquid preservation, Modena extender, sperm viability
In the swine industry, more than 99% of artificial inseminations (AIs) performed around the world today with extended liquid semen (Wagner and Thibier, 2000) whether on the same day or stored at 15°-20°C for 1-5 days before AI (Johnson et al., 2000). Success of A.I. depends on preservation of semen for longer period of time without reducing the fertility. Boar sperm is more sensitive to storage at cool temperatures than the sperms of other domestic species. Fertility of boar spermatozoa decreases with increase in storage time during liquid preservation. During storage, the boar spermatozoa undergo several changes including diminished motility, viability and alterations in membrane permeability. The aim of this study was to assess the effect of time of storage and the dynamic changes in sperm cell characteristics in normospermic boar semen stored in long-term extender, in order to determine the susceptibility to damage of particular structures of spermatozoa during cooling and storage at 15 °C for 120 hours of post collection.
MATERIALS AND METHODS
A total of 24 ejaculates, six from each four trained healthy crossbred Hampshire boars (Hampshire x Indigenous) of one to two years of age maintained at ICAR-All India Coordinated Research Project (AICRP) on Pig, Assam Agricultural University, Guwahati, Assam, India were selected for the present study. The semen was collected twice weekly by Simple fist method (Tamuli, 1994). Immediately after collection, the semen samples were evaluated for...