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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Antitumor applications of ascorbic acid (AA) and its oxidized form dehydroascorbic acid (DHA) can be quite challenging due to their instability and sensitivity to degradation in aqueous media. To overcome this obstacle, we have synthesized solid lipid nanoparticles loaded with ascorbyl palmitate (SLN-AP) with variations in proportions of the polymer Pluronic F-68. SLNs were synthesized using the hot homogenization method, characterized by measuring the particle size, polydispersity, zeta potential and visualized by TEM. To investigate the cellular uptake of the SLN, we have incorporated coumarin-6 into the same SLN formulation and followed their successful uptake for 48 h. We have tested the cytotoxicity of the SLN formulations and free ascorbate forms, AA and DHA, on HEK 293 and U2OS cell lines by MTT assay. The SLN-AP in both formulations have a cytotoxic effect at lower concentrations when compared to ascorbate applied the form of AA or DHA. Better selectivity for targeting tumor cell line was observed with 3% Pluronic F-68. The antioxidative effect of the SLN-AP was observed as early as 1 h after the treatment with a small dose of ascorbate applied (5 µM). SLN-AP formulation with 3% Pluronic F-68 needs to be further optimized as an ascorbate carrier due to its intrinsic cytotoxicity.

Details

Title
Synthesis and In Vitro Characterization of Ascorbyl Palmitate-Loaded Solid Lipid Nanoparticles
Author
Ledinski, Maja 1   VIAFID ORCID Logo  ; Marić, Ivan 2   VIAFID ORCID Logo  ; Štefanić, Petra Peharec 1   VIAFID ORCID Logo  ; Ladan, Iva 3 ; Katarina Caput Mihalić 1 ; Jurkin, Tanja 2 ; Gotić, Marijan 4 ; Urlić, Inga 1   VIAFID ORCID Logo 

 Faculty of Science, Division of Molecular Biology, Department of Biology, University of Zagreb, 10 000 Zagreb, Croatia; [email protected] (M.L.); [email protected] (P.P.Š.); [email protected] (K.C.M.) 
 Division of Materials Chemistry, Radiation Chemistry and Dosimetry Laboratory, Ruđer Bošković Institute, 10 000 Zagreb, Croatia; [email protected] (I.M.); [email protected] (T.J.) 
 Croatian Institute of Public Health, Department of Health Ecology, 10 000 Zagreb, Croatia; [email protected] 
 Division of Materials Physics, Laboratory for Molecular Physics and Synthesis of New Materials, Ruđer Bošković Institute, 10 000 Zagreb, Croatia; [email protected] 
First page
1751
Publication year
2022
Publication date
2022
Publisher
MDPI AG
e-ISSN
20734360
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2663100780
Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.