Content area
Abstract
Prime editors, which are CRISPR–Cas9 nickase (H840A)–reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%.
Prime editors are optimized for use in plants.
Details
Title
Prime genome editing in rice and wheat
Author
Lin Qiupeng 1 ; Zong Yuan 2 ; Xue Chenxiao 1 ; Wang, Shengxing 2 ; Jin Shuai 1 ; Zhu Zixu 1 ; Wang, Yanpeng 1 ; Anzalone, Andrew V 3 ; Raguram Aditya 3 ; Doman, Jordan L 3
; Liu, David R 3
; Gao Caixia 1
1 Innovation Academy for Seed Design, Chinese Academy of Sciences, State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Beijing, China (GRID:grid.9227.e) (ISNI:0000000119573309); University of Chinese Academy of Sciences, College of Advanced Agricultural Sciences, Beijing, China (GRID:grid.410726.6) (ISNI:0000 0004 1797 8419)
2 Innovation Academy for Seed Design, Chinese Academy of Sciences, State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Beijing, China (GRID:grid.9227.e) (ISNI:0000000119573309)
3 Broad Institute of Harvard and MIT, Merkin Institute of Transformative Technologies in Healthcare, Cambridge, USA (GRID:grid.66859.34); Harvard University, Department of Chemistry and Chemical Biology, Cambridge, USA (GRID:grid.38142.3c) (ISNI:000000041936754X); Harvard University, Howard Hughes Medical Institute, Cambridge, USA (GRID:grid.38142.3c) (ISNI:000000041936754X)
; Liu, David R 3
; Gao Caixia 1
1 Innovation Academy for Seed Design, Chinese Academy of Sciences, State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Beijing, China (GRID:grid.9227.e) (ISNI:0000000119573309); University of Chinese Academy of Sciences, College of Advanced Agricultural Sciences, Beijing, China (GRID:grid.410726.6) (ISNI:0000 0004 1797 8419)
2 Innovation Academy for Seed Design, Chinese Academy of Sciences, State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Beijing, China (GRID:grid.9227.e) (ISNI:0000000119573309)
3 Broad Institute of Harvard and MIT, Merkin Institute of Transformative Technologies in Healthcare, Cambridge, USA (GRID:grid.66859.34); Harvard University, Department of Chemistry and Chemical Biology, Cambridge, USA (GRID:grid.38142.3c) (ISNI:000000041936754X); Harvard University, Howard Hughes Medical Institute, Cambridge, USA (GRID:grid.38142.3c) (ISNI:000000041936754X)
Pages
582-585
Publication year
2020
Publication date
May 2020
ISSN
10870156
e-ISSN
15461696
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2476738573
Copyright
© The Author(s), under exclusive licence to Springer Nature America, Inc. 2020.