Epieriocalyxin A Induces Cell Apoptosis Through JNK and ERK1/2 Signaling Pathways in Colon Cancer Cells

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Web End = Cell Biochem Biophys (2015) 73:559564

DOI 10.1007/s12013-015-0687-4

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Web End = Epieriocalyxin A Induces Cell Apoptosis Through JNK and ERK1/2 Signaling Pathways in Colon Cancer Cells

Zhou Wang Zhijie Xu Zhengchuan Niu

Benjia Liang Jun Niu

Published online: 24 May 2015 Springer Science+Business Media New York 2015

Abstract Colorectal cancer is one of the most commonly diagnosed cancers in the world. Currently, drug resistance of cancer cell to chemotherapy is a major cause for cancer recurrence and death of the patients; therefore, new therapeutic strategy is required to improve the care of colorectal cancer patients. The Chinese herb, Isodon eriocalyx, has been used a therapeutic for a long time in China. In this study, we showed that Epieriocalyxin A (EpiA), a diterpenoid isolated from I. eriocalyx, suppressed Caco-2 colon cancer cell growth. EpiA induced annexin V ipping in cell membrane and DNA fragment. We also showed that EpiA induced the generation of ROS in cells, as well as damage of the mitochondrial membrane. Western blot results showed that both JNK and ERK1/2 activation was decreased after EpiA treatment in a dose-dependent manner. EpiA increased the expression of caspase 3 and Bax, and decreased Bcl2 expression. Our results suggest that EpiA is a novel compound that induces colon cancer apoptosis. EpiA could be a potential drug for colon cancer therapy in the future.

Keywords Epieriocalyxin A Colorectal cancer

Cell apoptosis Signaling pathway

Introduction

Colorectal cancer is the third most commonly diagnosed cancer in males and the second in females [1]. Surgical re-section remains the current primary treatment, followed by chemotherapy as adjuvant therapies. However, drug resistance of cancer cell to chemotherapy is still the major challenge, resulting in poor disease outcomes for the patients. Therefore, new therapeutic strategy for patients is still urgently needed.

Although herbal medicines have been used to treat patients in East Asian countries for thousands of years, recent years have witnessed renewed interest in herbal medicines as potential alternative therapies for the treatment of various cancers. The Chinese plant Isodon eriocalyx has been used...