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Abstract
Objective: The present study was carried out to evaluate the anticancer property of ethanol extract of Scaevola taccada leaves against Ehrlich ascites carcinoma in Swiss albino mice.
Materials and Methods: The leaves powder was subjected to continuous hot extraction using ethanol and cold maceration by water to get Ethanol and Aqueous extracts, respectively. Identification of the chemical constituents of plant extract was determined by standard procedures. The in vivo anticancer study was determined in mice using Ehrlich ascites carcinoma cell line. In vivo cytotoxic effect of the extract was assessed by monitoring the mean survival time, effect on hematological parameters, cell count, tumor weight and status of antioxidant enzymes such as lipid peroxidase, reduced glutathione, superoxide dismutase and catalase activities.
Results: In phytochemical screening, the crude extract revealed the presence of different chemical groups like alkaloids, saponins, steroids, flavonoids and glycosides. The extract showed a significant increase in the life span and a decrease in cancer cell number, tumor weight and tumor volume. The protective effect of the extract on the hemopoietic system at the dose level 200 and 400 mg/kg were noted. The extract effectively suppressed the elevated levels of serum glutamate pyruvate transaminase, serum glutamate oxaloacetate transaminase and alkaline phosphatase. The extract prevented lipid peroxidation and restored the antioxidant enzymes.
Conclusion: From the result it can be found that the ethanol extract of scaevola taccada Roxb showed significant (p<0.001) in vivo cytotoxic effect when compared to the tumor control group.
Key words: Scaevola taccada Roxb, In vivo Cytotoxicity studies, Ehrlich ascites carcinoma (EAC) cell line.
INTRODUCTION
The Plant Scaevola taccada Roxb belongs to the family Goodeniaceae, in vernacular language it is known as vellamuttagam, found in sea coasts of all around India and in the Andaman Islands. The Ethno pharmacology of the leaves revealed the uses of digestive, carminative, antitumor and anti-inflammatory properties. Fruit juice internally used to induce menstruation. The roots are used for dysentery. A decoction of the leaves and the bark was reported to combat tachycardia, one of the principal symptoms of beriberi. The drug reduces the frequency of heart beat, slow down the pulse rate and stimulates the heart for normal contraction. It exhibits diuretic property by increasing the tension in the renal arteries without causing...
