A Possible Relationship Between Polymorphisms of Glutathione S-Transferase M1, P1 and T1 Genes and Rheumatoid Arthritis in Zahedan, Southeast Iran/Güneydogu Iran Zahedan'da Glutation S-Transferaz M1, P1 ve T1 Genlerinin Polimorfizmleri ve Romatoid Artrit Arasindaki Muhtemel Iliski

Headnote

Objectives: This study aims to investigate the possible relationship between glutathione S-transferase (GST) gene (GSTM1, GSTT1, and GSTP1) polymorphisms and susceptibility to rheumatoid arthritis (RA).

Patients and methods: This case-control study was conducted on 100 Iranian patients with RA (88 females, 12 males; mean age 44.1±13.2 years; range 17 to 75 years) and 100 healthy subjects (68 females and 32 males; mean age 45.7±9.6 years; range 23 to 70 years). The GSTP1 polymorphism was determined using a tetra amplification refractory mutation system-polymerase chain reaction (Tetra-ARMS-PCR) assay, whereas the GSTM1 and GSTT1 polymorphisms were analyzed by multiplex PCR.

Results: The null genotype of GSTM1 was significantly higher in the patients with RA (78.0%) than in the control group (59.0%), and there was a risk factor for susceptibility to RA (OR=2.86, 95% CI=1.45-5.66, p=0.002). However, no significant difference was observed in the null genotype of GSTT1 among RA patients and healthy subjects (19% and 11%, respectively). There was no difference in the frequency distribution of the GSTP1 Ile105Val polymorphism between the groups (chi-square=1.69, p=0.429).

Conclusion: Our findings showed that only the GSTM1 genetic polymorphism is associated with RA risk in a sample of the Iranian population.

Key words: Glutathione S-transferase; polymorphism; rheumatoid arthritis.

Amaç: Bu çalismada glutation S-transferaz (GST) gen (GSTM1, GSTT1 ve GSTP1) polimorfizmi ve romatoid artrit (RA) yatkinligi arasindaki iliskinin arastirilmasi amaçlandi.

Hastalar ve yöntemler: Bu olgu kontrollü çalismaya Iranli 100 RA hastasi (88 kadin, 12 erkek; ort. yas 44.1±13.2 yil; dagilim 17-75 yil) ve 100 saglikli birey (68 kadin ve 32 erkek, ort. yas 45.7±9.6 yil; dagilim 23-70 yil) dahil edildi. GSTP1 polimorfizm tetra amplifikasyona dirençli mutasyon sistem polimeraz zincir reaksiyonu (Tetra-ARMS-PCR) ile belirlenirken, GSTM1 ve GSTT1 polimorfizmleri multipleks PCR kullanilarak analiz edildi.

Bulgular: GSTM1'in sifir genotipi, kontrol grubuna kiyasla (%59.0), RA'li hasta grubunda (%78.0) anlamli düzeyde daha yüksek olup, RA yatkinligi için bir risk faktörüydü (OR=2.86, %95 GA=1.45-5.66, p=0.002). Bununla birlikte, RA'li hastalar ve saglikli kisiler arasinda GSTT1'in sifir genotipi açisindan anlamli bir farka rastlanmadi (sirasiyla %19 ve %11). GSTP1 Ile105Val polimorfizminin siklik dagilimi gruplar arasinda farkli degildi (ki-kare=1.69, p=0.429).

Sonuç: Bulgularimiz yalnizca GSTM1 genetik polimorfizminin Iranli bir nüfus örnekleminde RA riski ile iliskili oldugunu göstermistir.

Anahtar sözcükler: Glutation S-transferaz; polimorfizm; romatoid artrit.

Rheumatoid arthritis (RA) is a chronic, systemic autoimmune...