Effect of 1,2-indanedione on PCR-STR typing of fingerprints deposited on thermal and carbonless paper

Abstract

1,2-Indanedione treated fingerprints deposited on two substrates (thermal and carbonless paper) were swabbed and tested to determine the effect of the chemical on PCR-STR DNA typing. Samples from 10 post-treatment intervals were analyzed. Two extraction methods, Chelex^® and Qiamp, were used to determine the effect of extraction procedure on the quality of the DNA profiles. The LCN DNA samples were concentrated using Microcon^® 100 and amplified using the Profiler Plus amplification kit. 1,2-Indanedione did not adversely affect the DNA profiles obtained from the treated fingerprints. Partial DNA profiles were obtained at all post-development time frames. Both extraction methods produced comparable profiles although more "drop-insâ[euro] were observed with the Qiamp method. More "drop-outsâ[euro] occurred in the DNA profiles from samples deposited on the thermal substrate compared with carbonless substrate.